Nextseq loading concentration. This technical note provides recommendations for Illumina DNA PCR-Free libraries across Illumina sequencing systems. Watch on Choosing a Library Loading Concentration for Illumina® Sequencing October 19, 2021 In this video, we explain how to choose a library loading concentration by first checking the library prep kit and sequencing platform documentation, then explaining how to use the average library size to determine a starting loading concentration. Entering the correct number of cycles in a read helps ensure optimum data output. The NextSeq 1000/2000 is compatible with all Illumina library preparation kits, but the optimal loading concentration can vary. . After library prep, libraries are diluted to the loading concentration appropriate for the library type, sequencing system, and reagent kit. Answer: Our recommendations for the optimal loading concentration, as well as pooling considerations, are listed in the sequencing section of each assay User Guide. Dilute Libraries to the Starting Concentration This step dilutes libraries to the starting concentration for your sequencing system and is the first step in a serial dilution. NextSeq 1000/2000 with XLEAP SBS kits loading guidance Illumina resources NextSeq 1000/2000 Specifications Sheet NextSeq 1000/2000 XLEAP-SBS and Standard SBS Concordance Tech Note Recommended library loading concentrations to obtain ideal cluster densities for all Illumina instruments and procedures to prevent over- under-clustering. CHMI owns and operates its own NextSeq 2000. ocu1 j6 bap5 lfbqbv ihvt cxc pyyp qv9ie ufo xekj